Two jobless Americans convince a prospector to travel to the mountains of Mexico with them in search of gold. But the hostile wilderness, local bandits, and greed all get in the way of their journey.
Dan Evans, a small time farmer, is hired to escort Ben Wade, a dangerous outlaw, to Yuma. As Evans and Wade wait for the 3:10 train to Yuma, Wade's gang is racing to free him.
One of the last bills signed by President Lincoln authorizes pushing the Union Pacific Railroad across the wilderness to California. But financial opportunist Asa Barrows hopes to profit from obstructing it. Chief troubleshooter Jeff Butler has his hands full fighting Barrows' agent, gambler Sid Campeau; Campeau's partner Dick Allen is Jeff's war buddy and rival suitor for engineer's daughter Molly Monahan. Who will survive the effort to push the railroad through at any cost?
Indian scout Tom Jeffords is sent out to stem the war between the American settlers and Apaches in the late 1870s Arizona. He learns that the Indians kill only to protect themselves, or out of retaliation for white atrocities.